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This study compares the effect of insulin given as a nasal spray with a placebo. Insulin is a chemical messenger hormone in the body that controls fat triglyceride levels in the blood by controlling the amount of fat made by the liver and gut. Recent research suggests that insulin may work through the brain.

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The investigators hypothesize that preferential delivery of insulin into the brain, through nasal spray of the hormone, may affect the amount of fats made by the liver and gut. Each subject will be studied twice weeks apart in random order in this single blinded study.

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In study A they will receive a single dose of insulin 40 IU through nasal spray. In study B they will receive placebo. On the day of the study subjects will drink regular liquid nutrient formula to maintain a constant fed state.

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A pancreatic clamp octreotide with replacement glucose, insulin and growth hormone will be started at 7am. Regular blood samples will be drawn to assess lipoprotein kinetics.

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Drug: Insulin Insulin 40 IU, nasal spray, single dose. Drug: Placebo Placebo, nasal spray, single dose. Triglyceride-rich lipoprotein apolipoprotein B production rate will be measured following nasal spray of insulin or placebo.

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Triglyceride-rich lipoprotein apolipoprotein B48 production rate will be measured following nasal spray of insulin or placebo. Any current or history of biliary disease including gall stones, biliary atresia and cholecystitis or pancreatitis. Any history of a myocardial infarction or clinically ificant, active, cardiovascular history including a history of arrhythmia's or conduction delays on electrocardiogram, unstable angina, or decompensated heart failure.

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Overall Status Completed. Collaborator none. Keywords provided by University Health Network, Toronto. Exploring the universe of clinical trials just got cosmically easier. Drug: Insulin Drug: Placebo.

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